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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | FC, ICC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 16-235 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488 |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488 is validated for use in FC, IC for the detection of phospho-erbB-2/HER-2 (Tyr1248). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic phosphopeptide, residues 1243-1255, corresponding to a major autophosphorylation (Tyr1248) site of human Erb B2/HER-2 (C-AENPEpYLGLDVPV) |
| Host | Rabbit |
| Specificity | the activated form of Erb B2/HER-2 oncoprotein |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Predicted to cross react with rat based on conservation of immunogenic sequence |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. |
| Gene Symbol |
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| Modifications |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin. SIZE: 1255 amino acids; 137910 Da SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity). SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 185kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunostaining of EGF treated A431 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at 4°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |