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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP | M | Gel Immobilized | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 16-219 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Myc Tag Antibody, clone 4A6, agarose conjugate |
| Background Information | Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. |
| Product Information | |
|---|---|
| Format | Gel Immobilized |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 covalently coupled to protein G agarose beads and provided as a 50% slurry in buffer containing PBS, pH 7.4, and 0.05% sodium azide. Liquid suspension. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myc Tag Antibody, clone 4A6, agarose conjugate detects level of Myc Tag & has been published & validated for use in IP. |
| Key Applications |
|
| Application Notes | Immunoaffinity Purification: A previous lot was used to purify Myc-tagged proteins. Elute with 100 mM tetraethyl ammonium (TEA), pH 11.5. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. |
| Clone | 4A6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Reactivity with other species has not been confirmed. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitts lymphomas, suggesting its importance in the normal function of this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5-CAC[GA]TG-3. Seems to activate the transcription of growth-related genes. SIZE: 439 amino acids; 48804 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | Varies |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoprecipitation of Myc-tagged Akt/PKB from a COS transfected cell lysate, subsequent detection using anti-Myc Tag (05-724). Immunoprecipitation Analysis: 4 μg of anti-Myc Tag, clone 4A6, agarose conjugate immunoprecipitated Myc-tagged Akt/PKB from 250 μg of a COS transfected cell lysate, which was then detected by immunoblot analysis, using 0.5 μg/mL of anti-Myc Tag, clone 4A6 (Catalog # 05-724). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |