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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | HRP | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 16-213 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Myc Tag Antibody, clone 4A6, HRP conjugate |
| Overview | Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. Species Cross-reactivity: Human, others not tested Ig Type: IgG Host: Mouse Molecular Weight: Varies depending upon the protein which is tagged |
| Alternate Names |
|
| Background Information | Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems. |
| Product Information | |
|---|---|
| Format | HRP |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon®, 0.2 mg/mL BSA) and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6. |
| Epitope | C-terminal |
| Clone | 4A6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Other species cross-reactivity not tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitts lymphomas, suggesting its importance in the normal function of this gene. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5-CAC[GA]TG-3. Seems to activate the transcription of growth-related genes. SIZE: 439 amino acids; 48804 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | Varies depending upon the protein which is tagged. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates. Western Blot Analysis: 1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |