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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Chp, Pm | IHC, ELISA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-066 |
| Description | Anti-ASPM Antibody |
| Alternate Names |
|
| Background Information | The ASPM gene is the human ortholog of the Drosophila melanogaster abnormal spindle gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ASPM Antibody detects level of ASPM & has been published & validated for use in IH, ELISA. |
| Key Applications |
|
| Application Notes | ELISA: Tested in direct ELISA against the ASPM immunogen. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human ASPM. |
| Epitope | N/A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes ASPM. |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with human. Predicted to react with chimpanzee and primate based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The ASPM gene is the human ortholog of the Drosophila melanogaster abnormal spindle gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Probable role in mitotic spindle regulation and coordination of mitotic processes By similarity. May have a preferential role in regulating neurogenesis. SUBCELLULAR LOCATION: Cytoplasm by similarity. Nucleus By similarity. Note: The nuclear-cytoplasmic distribution could be regulated by the availability of calmodulin by similarity. INVOLVEMENT IN DISEASE: Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5) [MIM:608716]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. SEQUENCE SIMILARITIES: Contains 2 CH (calponin-homology) domains. Contains 39 IQ domains. |
| Molecular Weight | 218, 95, 75 kDa Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kDa and Isoform 2 at 217.794kDa. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155–2165. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry Analysis: 1:300 dilution of this antibody detected ASPM in human ductal carcinoma tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |