Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Pm, B, Ch, Eq, Zebrafish | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-722 |
| Trade Name |
|
| Description | Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody |
| Alternate Names |
|
| Background Information | The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified rabbit polyclonal IgG in PBS with 0.05% NaN3 and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide encompassing |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1054 (human). |
| Species Reactivity |
|
| Species Reactivity Note | Human tested. Expected to react with Mouse, Rat, primate, cow, chicken, horse, and Danio based on 100% sequnce homolgy. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Double affinity purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1356 amino acids; 151527 Da SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. ... |
| Molecular Weight | 152 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: This lot detected VEGFR2 phosphorylated on Tyr1054 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |