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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-702 |
| Replaces | 04-1007 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-FOXO3a/FKHRL1 Antibody |
| Alternate Names |
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| Background Information | The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A. |
| Epitope | a.a. 330-673 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human and mouse. Not tested on other species. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O43524 # Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5-[AG]TAAA[TC]A-3. SIZE: 673 amino acids; 71277 Da SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. TISSUE SPECIFICITY: Ubiquitous. PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesnt appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.DISEASE:SwissProt: O43524 # A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. SIMILARITY: SwissProt: O43524 ## Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | Approx. 90-95 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on 3T3/A31 cell lysates. Western Blot Analysis: A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |