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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, Ca | ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-432 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-K+/Cl- Cotransporter (KCC2) Antibody |
| Alternate Names |
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| Background Information | KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABAs inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Liquid at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2) with more than 15 product citations. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution. |
| Biological Information | |
|---|---|
| Immunogen | N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Rat and dog. Wide range of species cross-reactivity expected due to sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9H2X9 # Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. SIZE: 1139 amino acids; 126184 Da SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain specific. Detected in neuronal cells. SIMILARITY:SwissProt: Q9H2X9 ## Belongs to the SLC12A transporter family. MISCELLANEOUS: Inhibited by furosemide and bumetanide. |
| Molecular Weight | 140 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot in rat brain membrane preperations. Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |