Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, Ch, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-307 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-STAT1 (Tyr701) Antibody |
| Alternate Names |
|
| Background Information | STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Immunoaffinity purified IgG in 0.02 M PBS containing 0.1% Sodium Azide + 50% Glycerol and 5 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-STAT1 (Tyr701) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-STAT1 (Tyr701) also known as Transcription factor ISGF-3 components p91/p84 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine. |
| Epitope | Tyr701 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Phosphorylated STAT1 |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, rat, and chicken. Other species cross-reactivity is unknown. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Immunoaffinity Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P42224 # Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. SIZE: 750 amino acids; 87335 Da SUBUNIT: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C, C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter (By similarity). Interaction with HCV core protein results in STAT1 degradation. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta. PTM: Phosphorylated on tyrosine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Serine phosphorylation is also required for maximal transcriptional activity in IFN-gamma transduction (lacking in beta form). DISEASE: SwissProt: P42224 # Defects in STAT1 are the cause of STAT1 deficiency [MIM:600555]. Patients generally suffer from mycobacterial or viral diseases. In the case of complete deficiency, patients can die of viral disease. & Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. SIMILARITY: SwissProt: P42224 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain. |
| Molecular Weight | 92 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot in RIPA lysates from CNTF-treated SK-N-MC cells. Western Blot Analysis: A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |