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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1720 |
| Replaces | MABE539; MABD181 |
| Description | Anti-FOXO4 Antibody |
| Alternate Names |
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| Background Information | Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the N-terminal region of FOXO4. |
| Epitope | N-terminus |
| Host | Rabbit |
| Specificity | This antibody recognizes FOXO4 at the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Tested on human and mouse. Predicted to react with rat based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 SUBUNIT: Interacts with CBP, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Ref.10 Ref.12 Ref.11 SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Ref.11 Ref.7 TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. PTM: Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. DISEASE: A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. Pharmaceutical use A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity. SIMILARITY: Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | ~65 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: 1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |