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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1708 |
| Description | Anti-FOXH1 Antibody |
| Alternate Names |
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| Background Information | Forkhead box H1 (FOXH1) is a member of the HNF–3FKH family of transcriptional activators. FOXH1 is a winged helix with two loops on the C-terminal side expressed in all human tissue. FOXH1 is an embryonic transcription regulator and is an essential transcriptional co-activator that binds with SMAD2 to activate activin, and therefore induce the gooseciod (GSC) promoter. After FOXH1 binds to SMAD4, activin response element is activated and TGF-β is stimulated. FOXH1 also interacts with NKX2-5 to form an essential component of anterior heart field development. FOXH1 is associated with complex brain malfunctions due to incomplete cleavage of the prosencephalon, and in developmental delays resulting in facial abnormalities such as cyclopia and severe cleft lip or palate. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic to peptide corresponding to FOXH1. |
| Host | Rabbit |
| Specificity | This antibody recognizes FOXH1. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with mouse based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION:Transcriptional activator. Recognizes and binds to the DNA sequence 5-TGT[GT][GT]ATT-3. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). Ref.1 SUBUNIT: Interacts with the MH2 domains of SMAD2 and SMAD3. Ref.1 SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. Ref.1 DOMAIN:The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex By similarity. Sequence similarities Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 38 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by immunocytochemistry on NIH/3T3, HeLa, and A431 cells. Immunocytochemistry: 1:500 dilution of this antibody was used to detect FOXH1 in NIH/3T3, HeLa and A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |