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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1562 |
| Replaces | 04-1047 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-EZH2 Antibody |
| Alternate Names |
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| Background Information | Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-EZH2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EZH2 also known as SET domain bifurcated 1, ERG-associated protein with SET domain. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, His-tagged fusion protein corresponding to residues 406-746 of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2). Based on accession # BC010858. |
| Host | Rabbit |
| Specificity | Recognizes EZH2, Mr ~100 kDa |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Gene Symbol |
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| Purification Method | Serum |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15910 # May be involved in the regulation of gene transcription and chromatin structure. SIZE: 746 amino acids; 85363 Da SUBUNIT: Binds ATRX via the SET domain (Probable). SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Expressed in many tissues. SIMILARITY: SwissProt: Q15910 ## Belongs to the EZ family. & Contains 1 SET domain. Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys-9 and Lys-27 of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A |
| Molecular Weight | 95-100kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot analysis |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |