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Millipore Use Anti-Jmjd4 (N-Terminus) Antibody (Rabbit Polyclonal Antibody) Validated In Wb, Icc, Elisa To Detect Jmjd4 (N-Terminus) Also Known As Jumonji Domain-Containing Protein 4. - Mill (Additional S&H or Hazmat Fees May Apply)
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed. JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues
Product Information
Format
Purified
Control
A431 lysate
Presentation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Quality Level
MQ100
Applications
Application
Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.
Key Applications
Western Blotting
Immunocytochemistry
ELISA
Application Notes
ELISA: This antibody was reported by an outside laboratory to be suitable for ELISA.
Immunocytochemistry: HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.
Biological Information
Immunogen
The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.
Epitope
N-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the N-terminal region of human JMJD4.
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_075383.1
Gene Symbol
FLJ12517
MGC129896
OTTHUMP00000035836
Purification Method
Protein G Chromatography
UniProt Number
Q9H9V9
UniProt Summary
SEQUENCE SIMILARITIES: Contains 1 JmjC domain. CAUTION: It is uncertain whether Met-1 or Met-47 is the initiator.
Molecular Weight
~53 kDa
Product Usage Statements
Quality Assurance
Western Blot Analysis: 1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
