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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Gt | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1386 |
| Description | Anti-Connective Tissue Growth Factor Antibody |
| Alternate Names |
|
| Background Information | Connective Tissue Growth Factor (CTGF) is a member of the CNN family of growth factors that are characterized as having cysteine-rich peptides with four specific domains. CTGF was originally identified in endothelial cells as a significant chemotactic and mitogenic factor. Research has revealed CTGF to have several biological properties which include chemotaxis, cell proliferation, promotion of adhesion and the formation of extracellular matrix. It is also necessary for such biological processes as wound healing, scar formation, fibrosis, embryogenesis and angiogenesis. In its role as an inducer of angiogenesis, CTGF has been associated with vascular endothelial growth factor (VEGF). As a result of its pro-fibrotic properties, CTGF is thought to be a promising target for therapy in conditions involving pathological fibrosis such as chronic fibrotic disease, chronic kidney disease and proliferative diabetic retinopathy (PDR). Cell types found to express CTGF are endothelial cells, fibroblasts and vascular smooth-muscle cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified goat polyclonal in buffer containing Tris-Glycine pH 8.0 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Connective Tissue Growth Factor Antibody detects level of Connective Tissue Growth Factor & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the mature chain of human Connective tissue growth factor. |
| Epitope | Mature chain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Goat |
| Specificity | This antibody recognizes the mature chain of Connective tissue growth factor. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Primate (98% sequence homolgy). Monkey (97% sequence homology). Porcine and Bovine (93% sequence homology). Rat and mouse (91% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. SUBCUNIT STRUCTURE: Monomer. SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix By similarity. Secreted By similarity. SEQUENCE SIMILARITIES: Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain. |
| Molecular Weight | In Western Blot, full length CTGF is observed at 38 kDa and CTGF N&C domain is observed at 19 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in untreated and TGFb1 treated NRK cell lysates. Western Blot Analysis: 5 µg/mL of this antibody detected Connective tissue growth factor on 10 µg of untreated and TGFb1 treated NRK cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |