| Replacement Information | |
|---|---|
| Replacement Information | 07-1224 is a recommended replacement for AB4091 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IP, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1224 |
| Replaces | AB4091 |
| Description | Anti-PP6C Antibody |
| Alternate Names |
|
| Background Information | This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P). |
| Key Applications |
|
| Application Notes | Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL. Immunohistochemistry(paraffin): Optimal Staining of PP6 Polyclonal Antibody: Human Testis PP6 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. Antibody was diluted 1:100, using IHC-Select® detection with HRP-DAB. |
| Biological Information | |
|---|---|
| Immunogen | The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. The N-terminal domain of this protein restricts G1 to S phase progression in cancer cells. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O00743 # May function in cell cycle regulation. COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity). SIZE: 305 amino acids; 35144 Da SUBCELLULAR LOCATION: Cytoplasm (By similarity). TISSUE SPECIFICITY: Highest expression levels in testis, heart, and skeletal muscle and lowest in placenta, lung and kidney. SIMILARITY: SwissProt: O00743 ## Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. |
| Molecular Weight | Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Jurkat lysates. Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |