| Replacement Information | |
|---|---|
| Replacement Information | 07-1218 is a recommended replacement for AB4084 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1218 |
| Replaces | AB4084 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PP1γ1 Antibody |
| Alternate Names |
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| Background Information | Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation. |
| Biological Information | |
|---|---|
| Immunogen | The immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323). |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P36873 # Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. COFACTOR: Binds 1 iron ion per subunit. & Binds 1 manganese ion per subunit. SIZE: 323 amino acids; 36984 Da SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with cyanobacterial toxin microcystin; disulfide-linked. Interacts with PPP1R7. Isoform gamma-2 interacts with SPZ1 (By similarity). Interacts with CDCA2. SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with SPZ1 in the nucleus (By similarity). SIMILARITY: SwissProt: P36873 ## Belongs to the PPP phosphatase family. PP-1 subfamily. MISCELLANEOUS: Microcystin toxin is bound to Cys-273 through a thioether bond. |
| Molecular Weight | ~37 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on HeLa lysates. Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |