| Replacement Information | |
|---|---|
| Replacement Information | 07-1210 is a recommended replacement for AB1622. |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Po, Rb, R, Ca | WB, IF, IH(P), ELISA, IP, RIA | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1210 |
| Replaces | AB1622 |
| Description | Anti-IP3 Receptor Antibody |
| Alternate Names |
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| Background Information | The InsP3 (inositol 1,4,5-trisphosphate) Receptor family are intracellular Ca2+ channels activated by the second messenger InsP3. There are at least three types of receptor, with distinct tissue-specific expression profiles and subcellular localizations; it is thought that the exact subtype profile conveys precise Ca2+ release responses. Release of Ca2+ from intracellular stores is the trigger for many cellular responses, including muscle contraction. Receptor activity is subject to feedback regulation by Ca2+ concentration, as high cytosolic Ca2+ concentrations shut down the channel. Ca2+ release is augmented by phosphorylation of the Receptor by PKA and PKG. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified rabbit polyclonal IgG in buffer containing 10 mM sodium phosphate, 150 mM NaCl, and 0.01% sodium azide, pH 7.5. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-IP3 Receptor Antibody is validated for use in WB, IF, IH(P), ELISA, IP, RIA for the detection of IP3 Receptor. |
| Key Applications |
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| Application Notes | Immunohistochemisty (paraffin): IP3 Receptor staining in rat cerebellum, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB. Immunofluorescence: 1:20 - 1:200 dilution from a previous lot. Light fixation, 2% PFA, 90% ethanol fixation; Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the IP3R. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended. ELISA: 1:2,000 dilution of a previous was used in ELISA. Immunoprecipitation: 1:200 dilution of a previous lot was used in Immunoprecipitation. Flow cytometry: 1:1,000 dilution of a previous lot was used in Flow Cytometry. Radioimmunoassay: 1:10,000 dilution of a previous lot was used in Radioimmunoassay. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide (KDSTEYTGPESYV) corresponding to the C-terminus of human IP3 Receptor type 1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Reacts with the C-terminal cytoplasmic domain of IP3 receptors Type 1, 2, and 3. IP3 receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation. AB1622 reacts with IP3 receptor (MW approx. 260 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues). |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Reacts with Bovine, Human, Mouse, Porcine (Pig), Rabbit, Rat and Canine (Dog). Reactivity with other species has not been determined. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q14643 # Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5- trisphosphate. SIZE: 2758 amino acids; 313945 Da SUBUNIT: Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with TXNDC4 in a pH-, redox state- and calcium- dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1 (By similarity). Interacts with MRVI1. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. DOMAIN: SwissProt: Q14643 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. PTM: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels (By similarity). & Phosphorylated on tyrosine residues. DISEASE: SwissProt: Q14643 # Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is a very slow progressing form. It has a wide range of onset from childhood to adult. Most patients remain ambulatory. SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains. MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium- binding protein which then inhibits the receptor |
| Molecular Weight | ~260 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on RAW 264 lysates. Western Blot Analysis: 1:500-1,000 dilution of this lot detected IP3 Receptor on 10 μg of RAW 264 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |