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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, B, Ca, Po, Rb | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-052 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Phospholamban (Ser16) Antibody |
| Alternate Names |
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| Background Information | Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Bovine. Predicted to cross-react with human, mouse, rat, rabbit, pig and dog. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. |
| Gene Symbol |
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| Modifications |
|
| Purification Method | Immunoaffinity Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. SIZE: 52 amino acids; 6109 Da SUBUNIT: Homopentamer. SUBCELLULAR LOCATION: Membrane. TISSUE SPECIFICITY: Heart. PTM: Phosphorylated in response to beta-adrenergic stimulation. DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function. SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family. |
| Molecular Weight | Varies |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA. Western Blot Analysis: 0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes. DO NOT BOIL CARDIAC PREPARATION. Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |