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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-900 |
| Replaces | 04-1071 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SynGAP Antibody |
| Background Information | Silent synapses, or excitatory synapses that lack functional -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), are thought to be critical for regulation of neuronal circuits and synaptic plasticity. SynGAP, an excitatory synapse-specific RasGAP, regulates AMPAR trafficking, silent synapse number, and excitatory synaptic transmission in hippocampal and cortical cultured neurons. Over expression of SynGAP in neurons results in a remarkable depression of AMPAR-mediated miniature excitatory postsynaptic currents, a significant reduction in synaptic AMPAR surface expression, and a decrease in the insertion of AMPARs into the plasma membrane. Synaptic transmission is increased in neurons from SynGAP knockout mice as well as in neuronal cultures treated with SynGAP small interfering RNA. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified in PBS with 0.05% NaN3 and 30% Glycerol |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SynGAP Antibody detects level of SynGAP & has been published & validated for use in IC & WB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: 0.1 µg/mL has been reported to immunostain SynGAP in neuron cultures fixed with 4% paraformaldehyde, 4% sucrose and perme-abilized with 0.1% Triton X-100 (2). |
| Biological Information | |
|---|---|
| Immunogen | peptide (KRLLDAQRGSFPPWVQQTRV) corresponding to amino acids 1274-1293 of rat SynGAP-a |
| Host | Rabbit |
| Specificity | SynGAP |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q96PV0 # Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR- mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits (By similarity). SIZE: 1343 amino acids; 148284 Da SUBUNIT: Interacts CAMK2A and CAMK2B (By similarity). Interacts with MPDZ. PTM: Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption (By similarity). SIMILARITY: SwissProt: Q96PV0 ## Contains 1 C2 domain. & Contains 1 PH domain. & Contains 1 Ras-GAP domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |