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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, WB, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-891 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SMRTe Antibody |
| Background Information | SMRT is 1495 amino acids and contains an 8 amino acid sequence that is not present in SMRTe (SMRT-extended), which contains 2514 amino acids. SMRTe contains an N-terminal sequence spanning over 1,000 amino acids that is not present in SMRT, but that shows significant similarity with N-CoR. SMRTe expression is regulated during cell cycle progression, suggesting a role for SMRTe in the regulation of cycle-specific gene expression in diverse signaling pathways. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SMRTe Antibody is a high quality Rabbit Polyclonal Antibody for the detection of SMRTe & has been validated in ChIP, ICC & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST fusion-protein corresponding to residues 1146-1349 of human SMRTe |
| Host | Rabbit |
| Specificity | SMRT isoforms |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9Y618 # Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. SIZE: 2517 amino acids; 274034 Da SUBUNIT: Interacts with HDAC7 (By similarity). Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. The SRMT isoform interacts with HDAC10. Interacts with MINT. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with CBFA2T3. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. High levels of expression are detected in lung, spleen and brain. DOMAIN: SwissProt: Q9Y618 The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor- interacting domains that are divided in two separate interaction domains (ID1 and ID2). & The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity. SIMILARITY: Belongs to the N-CoR nuclear receptor corepressors family. & Contains 2 SANT domains. |
| Molecular Weight | 270kDa, 180kDa and 80kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on nuclear fraction from murine brain |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |