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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ICC | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-429-I |
| Description | Anti-IGF-IRα subunit Antibody |
| Alternate Names |
|
| Background Information | Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate. Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain. |
| Epitope | Third Fibronectin Type III (FnIII 3) domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Chicken |
| Isotype | IgY |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~110/180 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected IGF-IRα subunit in 10 µg of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |