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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, H, M | ICC, IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-225 |
| Replaces | 04-210 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-c-Jun Antibody |
| Alternate Names |
|
| Background Information | c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Whole rabbit antiserum containing 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect c-Jun, also known as transcription factor AP-1, with Anti-c-Jun rabbit polyclonal antiserum, Cat. No. 06-225, that has been demonstrated to work in Immunocytochemistry, Immunoprecipitation, and Western blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Trp E fusion-protein containing the DNA binding domain of avian c-Jun |
| Host | Rabbit |
| Specificity | c-Jun |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Gene Symbol |
|
| Purification Method | Antiserum |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05412 # Transcription factor that recognizes and binds to the enhancer heptamer motif 5-TGA[CG]TCA-3. SIZE: 331 amino acids; 35676 Da SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC. SIMILARITY: SwissProt: P05412 ## Belongs to the bZIP family. Jun subfamily. & Contains 1 bZIP domain. |
| Molecular Weight | 35-39 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from human A431 cells or in Jurkat cell lysate |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |