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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1425 |
| Description | Anti-phospho-TAK1 (Ser412) Antibody |
| Alternate Names |
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| Background Information | TAK1 MAPKKK is activated by TAB1 to transform growth factor-beta (TGF-beta) signaling. TAK1 can also associate with TAB1 to form a unique MAPKKK complex that is thought to activate AP-1 and the NFkappaB signaling pathways. TAK1 has been linked to both IL-1alpha and TNFalpha signaling and therefore may also be involved in the inflammatory response. Recent research has found that the TAK1-TAB1 complex may have anti-inflammatory effects by aiding stercurensin in regulating the NFκB-dependent inflammatory pathways. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-TAK1 (Ser412) using this Anti-phospho-TAK1 (Ser412) Antibody validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human TAK1 phosphorylated at Ser412. |
| Epitope | Phosphorylated Ser412 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes TAK1 phosphorylated at Ser412. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. |
| Gene Symbol |
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| Modifications |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF- kappa-B activation and the p38 MAPK pathway. COFACTOR: Magnesium. SIZE: 606 amino acids; 67196 Da SUBUNIT: Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2. Interacts with PPM1L. PTM: Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation. SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | ~70 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in lambda phosphatase treated and untreated Murine RAW cell lysates. Western Blot Analysis: 1 µg/mL of this antibody detected TAK1 on 10 µg of lambda phosphatase treated and untreated Murine RAW cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |