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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1125 |
| Description | Anti-Ipaf (NLRC4) Antibody |
| Alternate Names |
|
| Background Information | Ipaf, also known as NLRC4, is a human CED-4 homologue and is thought to be an activator of pro-caspase-1 and might also be associated with the activation of caspase-1 as a response to pro-inflammatory and apoptotic stimulus. It has been shown to be induced by p53 and contributes to p53-dependent apoptosis. Ipaf is thought to play a role in the activation of the innate immune system. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Ipaf (NLRC4) Antibody is validated for use in IC, WB for the detection of Ipaf (NLRC4). |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 ug/ml of this antibody detected Ipaf in 10 ug of human lung tissue lysate. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ipaf in NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Ipaf (NLRC4) at the LRR4 domain. |
| Epitope | LRR4 domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes Ipaf at the LRR4 domain. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Summary: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a role in the promotion of apoptosis. SUBUNIT STRUCTURE: Self-associates and binds to ASC, pro-caspase-1, NOD2, BCL10 and NALP1 (NAC) by CARD-CARD interaction. SUBCELLULAR LOCATION: Cytoplasm. Note: Cytoplasmic filaments. TISSUE SPECIFICITY: Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. SEQUENCE SIMILARITIES: Contains 1 CARD domain. Contains 14 LRR (leucine-rich) repeats. Contains 1 NACHT domain. |
| Molecular Weight | ~112 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human lung tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of human lung tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |