Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Chp, Rhesus Macaque, Pm | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1115 |
| Description | Anti-Interleukin-18 (IL-18) Antibody |
| Alternate Names |
|
| Background Information | IL-18 has been shown to be a pro-inflammatory cytokine that induces interferon-gamma. IL-18 has also been found to have the ability to activate nuclear factor-kappaB (NFkB), express Fas ligand, induce CC and CXC chemokines, and also has been found to increase the production of viable human immunodeficiency virus. IL-18 has been linked to syndromes displaying symptoms of chronic low-levels of inflammation. IL-18 also seems to have some control over homeostasis and behavior in the central nervous system as well as neuroinflammatory activity. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Interleukin-18 (IL-18) Antibody is validated for use in WB, IH(P) for the detection of Interleukin-18 (IL-18). |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected IL-18 in 10 µg of human tonsil tissue lysate. Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected IL-18 in normal human spleen tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Interleukin-18. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with chimpanzee, rhesus macaque, and primate based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. SUBCELLULAR LOCATION: Secreted. SEQUENCE SIMILARITIES: Belongs to the IL-1 family. |
| Molecular Weight | ~22 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human tonsil tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-18 in 10 µg of human tonsil tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |