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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ca, M, R | WB, ICC, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1002 |
| Description | Anti-LAP2 Antibody |
| Alternate Names |
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| Background Information | The inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A. The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF. |
| Key Applications |
|
| Application Notes | Immunofluorescence: Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence. Immunocytochemistry: Representative lot data. A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic linear peptide corresponding to LAP2. |
| Host | Rabbit |
| Specificity | This antibody recognizes LAP2. |
| Species Reactivity |
|
| Species Reactivity Note | Tested on human and mouse. Predicted to cross-react with canine (dog) and rat based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide. SUBUNIT STRUCTURE: Interacts with LMNB1, LMNB2, BANF1, NAKAP95, GMCL and chromosomes By similarity. SUBCELLULAR lOCATION: Nucleus inner membrane; Single-pass type II membrane protein. Note= Tightly associated with the nuclear lamina. TISSUE SPECIFICTY: Expressed in many tissues. Most abundant in adult thymus and fetal liver. DOMAIN: Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1. PTM: Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes By similarity. PHARMACEUTICAL USE: TP5 is available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Used in primary and secondary immune deficiencies, autoimmunity, infections and cancer. SIMILARITY: Belongs to the LEM family. Contains 1 LEM domain. Contains 1 LEM-like domain. |
| Molecular Weight | ~55 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on HeLa cell lysate. Western Blot Analysis: A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |