| Replacement Information | |
|---|---|
| Replacement Information | 05-947 is a recommended replacement for MAB3080 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | ELISA, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-947 |
| Replaces | MAB3080 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Phosphotyrosine Antibody, clone PY20 |
| Background Information | Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins. ELISA: Recommended by an independent laboratory. |
| Biological Information | |
|---|---|
| Immunogen | Phosphotyrosine conjugated to carrier protein. |
| Clone | PY20 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Tyrosine-phosphorylated proteins from all species. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Species Reactivity Note | Most common vertebrate species tested. Reactivity with other species has not been confirmed. |
| Antibody Type | Monoclonal Antibody |
| Modifications |
|
| Purification Method | Protein A Purfied |
| Molecular Weight | Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot with RIPA lysates EGF-treated human A431 carcinoma cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of recipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application. |
| Packaging Information | |
|---|---|
| Material Size | 1 mg |