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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | Rb | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-822 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal |
| Product Information | |
|---|---|
| Format | Purified |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rho (-A Antibody, -B, -C), clone 3L74 is an antibody against Rho (-A, -B, -C) for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility |
| Clone | clone 3L74 |
| Host | Rabbit |
| Specificity | Recognizes Rho (-A, -B, -C) |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Broad species cross-reactivity expected based on sequence homology |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. COFACTOR: Magnesium. SIZE: 193 amino acids; 21768 Da SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. SUBCELLULAR LOCATION: Membrane; Lipid-anchor. DOMAIN: SwissProt: P61586 The basic-rich region is essential for yopT recognition and cleavage. PTM: Substrate for botulinum ADP-ribosyltransferase. & Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage. SIMILARITY: Belongs to the small GTPase superfamily. Rho family. |
| Molecular Weight | Mr 22kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 2 years at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |