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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, Ht, M | IP, WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-813 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-HDAC3 Antibody, clone 3G6 |
| Background Information | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-HDAC3 Antibody, clone 3G6 is a Mouse Monoclonal Antibody for detection of HDAC3 also known as histone deacetylase 3 & has been tested in IP, WB, ICC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide (NEFYDGDHDNDKESDVEI) corresponding to amino acids 411-428 of human HDAC3 |
| Clone | clone 3G6 |
| Host | Mouse |
| Specificity | HDAC3 |
| Isotype | IgG2a |
| Species Reactivity |
|
| Species Reactivity Note | Predicted to cross-react with rat and chicken based on sequence homology |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O15379 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. SIZE: 428 amino acids; 48848 Da SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 (By similarity). Interacts with CBFA2T3. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed. PTM: Sumoylated in vitro. SIMILARITY: SwissProt: O15379 ## Belongs to the histone deacetylase family. Type 1 subfamily. |
| Molecular Weight | 49-53kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoprecipitation/HDAC |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |