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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-673 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-Acetyl CoA Carboxylase (Ser79) using this Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody, clone BK102 validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to rat Acetyl CoA Carboxylase |
| Clone | BK102 |
| Host | Mouse |
| Specificity | Recognizes phosphorylated Acetyl CoA Carboxylase (Ser79). |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene. |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. COFACTOR: Biotin. & Binds 2 manganese ions per subunit. SIZE: 2346 amino acids; 265554 Da SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1. SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain. |
| Molecular Weight | 257 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on HeLa acid extracts |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |