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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Xn | IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-596 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Cdk2 Antibody, clone AN4.3 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cdk2 with Anti-Cdk2 Antibody, clone AN4.3 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB. |
| Key Applications |
|
| Application Notes | 0.5-2μg/ml of this lot detected cdk2 in HeLa nuclear extract. 4μg of a previous lot immunoprecipitated cdk2 from 500μg of mouse 3T3 nuclear extract. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human cdk2 |
| Clone | clone AN4.3 |
| Host | Mouse |
| Specificity | cdk2, does not react with other cdks |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. |
| Gene Symbol |
|
| Purification Method | Protein G Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. SIZE: 298 amino acids; 33930 Da SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1. SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 33kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on HeLa nuclear extract |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |