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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Po | WB, IP, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-584 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Tie2/TEK Antibody, clone Ab33 |
| Alternate Names |
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| Background Information | TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory. Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections. This antibody is not suitable for paraffin-embedded sections. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33 |
| Clone | clone Ab33 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Tie2/TEK, Mr 145kDa |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q02763 # This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation. SIZE: 1124 amino acids; 125811 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney. DISEASE:SwissProt: Q02763 # Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels. SIMILARITY: SwissProt: Q02763 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Molecular Weight | 145 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on RIPA lysates from HUVEC cells. Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |