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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-413 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F |
| Alternate Names |
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| Background Information | Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20ºC. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F is validated for use in WB for the detection of phospho-GSK3 (Tyr279/Tyr216). |
| Key Applications |
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| Applications Not Recommended |
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| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide (C-KQLLHGEPNVS[pY]ICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme coupled to ovalbumin. |
| Epitope | Surrounding pTyr214 (Drosophila GSK-3), pTyr279 (human GSK3α), pTyr216 (human GSK3β) |
| Clone | 5G-2F |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes GSK-3 phosphorylated at the target tyrosine residue (human GSK-3α pY279 and GSK-3β pY216). The 17-amino acid immunogen peptide sequence differs from the corresponding human, mouse, and rat sequence by only two amino acid residues. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, and rat. Predicted to react with GSK-3 orthologs from drosophila melanogaster and many other species based on sequence homology surrounding the target phosphotyrosine residue. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila shaggy (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM] |
| Gene Symbol |
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| Modifications |
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| Purification Method | Protein G Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity). SIZE: 483 amino acids; 50981 Da SUBUNIT: Monomer (By similarity). SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 51 kDa (alpha) and 46 kDa (beta) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on human Jurkat cells, mouse 3T3/A31, human A431, or rat L6 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected phospho-GSK3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK3 in mouse 3T3/A31, human A431 lysates and rat L6 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |