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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt, M, R, H, Di, Yeast (S. pombe) | ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-412 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-GSK3 Antibody, clone 4G-1E |
| Alternate Names |
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| Background Information | Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein G purified mouse IgG1 in storage buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GSK3 Antibody, clone 4G-1E detects level of GSK3 & has been published & validated for use in IC & WB. |
| Key Applications |
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| Applications Not Recommended |
|
| Application Notes | Immunoprecipitation: Not recommended. Immunocytochemistry: 10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone. |
| Biological Information | |
|---|---|
| Immunogen | 18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E. |
| Clone | 4G-1E |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | GSK3, α and β isoforms |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, rat, S. pombe, Dictyostelium and human. Because of the extremely high conservation of this immunogen among the known enzymes of many different species, this antibody is predicted to cross-react with nearly all species homologues. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila shaggy (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM] |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity). SIZE: 483 amino acids; 50981 Da SUBUNIT: Monomer (By similarity). SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein |
| Molecular Weight | 51 kDa (α) or 46 kDa (β) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on human Jurkat cells, human A431, rat L6, rat PC12 or mouse 3T3 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |