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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B | ICC, IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-390 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-erbB-3/HER-3 Antibody, clone 2F12 |
| Alternate Names |
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| Background Information | The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA, with 0.09% sodium azide. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect erbB-3/HER-3 using this Anti-erbB-3/HER-3 Antibody, clone 2F12 validated for use in IC, IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 4 µg of a previous lot of this antibody immunoprecipitated erbB-3/HER-3 from human MDA and MCF-7 RIPA lysates. Immunocytochemistry: 10 µg/mL of a previous lot of this antibody gave positive immunostaining for erbB-3/HER-3 in MCF-7cells fixed with 95% ethanol/5% acetic acid. Immunohistochemistry: 1:50 and 1:100 dilution of a previous lot detected erbB-3/HER-3 membrane staining in human normal and carcinoma breast tissue. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. The epitope maps between amino acid residues 1295-1323 of human erbB-3. Clone 2F12. |
| Epitope | a.a. 1295-1323 of human erbB-3 |
| Clone | 2F12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes erbB-3/HER-3, Mr 185 kDa. Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to cross-react with mouse, rat, and cow. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. |
| Gene Symbol |
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| Purification Method | Protein A chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK. SIZE: 1342 amino acids; 148098 Da SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Epithelial tissues and brain. DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity). DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 185 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on RIPA lysates of human MCF-7 cells. Western Blot Analysis: 0.1-2 µg/mL of this lot detected erbB-3/HER-3 in RIPA lysates of human MCF-7 cells. Previous lots have detected erbB-3/HER-3 in MDA RIPA lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |