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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1567 |
| Replaces | 04-237 |
| Description | Anti-p120 Catenin Antibody, clone 15D2 |
| Alternate Names |
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| Background Information | p120 Catenin is a cadherin-associated protein that binds intracellular proteins, such as the small GTPase, RhoA, and controls E-cadherin funcation and stability. Proteins of the p120 family and their isoforms are expressed in cells via alternative splicing, and all of them are essential for HGF signaling to Rac1. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-p120 Catenin Antibody, clone 15D2 (mouse monoclonal antibody) validated in WB, ICC, FC to detect p120 Catenin also known as Cadherin-associated Src substrate ,catenin (cadherin-associated protein) delta 1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fusion protein derived from the C-terminus of murine p120 |
| Epitope | C-terminus |
| Clone | clone 15D2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes p120 Catenin |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human and Mouse. Predicted to react with Rat based on homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway By similarity. May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. SUBUNIT STRUCTURE: Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. Binds to the C-terminal fragment of PSEN1 and mutually competes for E-cadherin. Interacts with ZBTB33. Interacts with GLIS2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membraneBy similarity. Note= Interaction with GLIS2 promotes nuclear translocation By similarity. TISSUE SPECIFICITY: Expressed in vascular endothelium. INDUCTION: Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure. DOMAIN: A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. PTM: Phosphorylated By similarity. INVOLVEMENT IN DISEASE: May contribute to cell malignancy. Complete loss of expression was observed in approximately 10% of invasive ductal breast carcinomas investigated. SEQUENCE SIMILARITIES: Belongs to the beta-catenin family. Contains 10 ARM repeats. |
| Molecular Weight | 90-120 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Hela cell lysate. Western Blot Analysis: 1 g/mL of this antibody detected p120 Catenin in lysate from Hela cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |