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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1466 |
| Description | Anti-FOXA1 Antibody, clone 2F83 |
| Alternate Names |
|
| Background Information | FOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER with chromatin (Badve, 2007). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha. |
| Key Applications |
|
| Application Notes | Immunohistochemisty: Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human GST-FOXA1 protein encompassing amino acids 7-86. |
| Epitope | N-terminus |
| Clone | 2F83 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody detects human FOXA1 (HNF3α). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human and Mouse. Other species have not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. SUBCELLULAR LOCATION: Nucleus. SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | 49 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on HeLa nuclear extracts. Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |