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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, WB, IH(P), IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1431 |
| Replaces | 04-1129 |
| Description | Anti-Casein Kinase 2α Antibody, 1AD9 |
| Alternate Names |
|
| Background Information | Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: Recommended Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only). Optimal working dilutions must be determined by end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant α-subunit of casein kinase 2. |
| Epitope | Region common to both alpha and alpha subunits |
| Clone | 1AD9 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. |
| Gene Symbol |
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| Purification Method | Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates Ser-392 of p53/TP53 following UV irradiation. SIZE: 391 amino acids; 45144 Da SUBUNIT: Tetramer composed of an alpha chain, an alpha and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1. SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain |
| Molecular Weight | 44 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |