| Replacement Information | |
|---|---|
| Replacement Information | 05-1406 is a recommended replacement for MAB1668 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1406 |
| Replaces | MAB1668 |
| Description | Anti-Phospholipase A2 Antibody, clone CH-7 |
| Alternate Names |
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| Background Information | Phospholipases hydrolyze phosphoglycerides to form smaller compounds which often serve as critical second messengers. Phospholipase A2 (PLA2) hydrolyzes phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation, adhesion, T-cell activation, and smooth muscle cell contractility. PLA2 isoforms include membrane-associated, Ca2+-independent forms, cytosolic, Ca2+-dependent forms, and secretory forms. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, to generate inositol (1,4,5) trisphosphate (IP3) and diacylglycerol (DAG), leading to Ca2+ release and PKC activation, respectively. At least four types of PLC have been identified. PLCβ, is activated by G-Protein-Coupled Receptors (GPCRs) and heterotrimeric G-Proteins. Mechanisms of PLCδ activation are unclear, but may also involve GPCRs. PLCγ, is activated by receptor tyrosine kinases, and appears essential for signaling by the PDGF Receptor. PLCε is a Guanine Nucleotide Exchange factor (GEF) for the small G-Protein Ras. PLD activity is up-regulated when cells are stimulated by hormones, growth factors or other extracellular signals. Two distinct PLDs, PLD1 and PLD2, have been identified. PLD hydrolyzes phosphatidylcholine to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis and cytoskeletal changes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P). |
| Key Applications |
|
| Application Notes | ELISA: A previous lot of this antibody was used in ELISA. Optimal working dilutions must be determined by the end user. Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant cPLA2 |
| Clone | CH-7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes PLA2. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM] |
| Gene Symbol |
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| Modifications |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P04054 # PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. COFACTOR: Binds 1 calcium ion per subunit (By similarity). SIZE: 148 amino acids; 16360 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY:SwissProt: P04054 ## Belongs to the phospholipase A2 family. |
| Molecular Weight | Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on RAW 264 lysates. Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at 2-8°C for 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |