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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-771 |
| Replaces | 04-406 |
| Description | Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal |
| Alternate Names |
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| Background Information | Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160). |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | Cultured supernantant containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes. |
| Epitope | a.a. 455-469 |
| Clone | AW114 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Akt2/PKBβ, Mr ~60 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human and rat. Predicted to cross-react with mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: General protein kinase capable of phosphorylating several known proteins. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation. SUBUNIT STRUCTURE: Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. TISSUE SPECIFICITY:In all human cell types so far analyzed. INVOLVEMENT IN DISEASE: Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
| Molecular Weight | ~60 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µl |