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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-539 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-MAPKAP-K2 Antibody, clone 7H4.2 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 200 µg (1 mg/mL) of mouse monoclonal IgG in PBS containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MAPKAP-K2 Antibody, clone 7H4.2 detects level of MAPKAP-K2 & has been published & validated for use in IP & WB. |
| Key Applications |
|
| Application Notes | Suggested dilutions: Immunoblot Analysis: 1:500 Immunoprecipitation: 1:20 |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2. |
| Clone | 7H4.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at Ser-7 and tyrosine hydroxylase (on Ser-19 and Ser-40). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. SIZE: 400 amino acids; 45568 Da SUBUNIT: Interacts with PHC2. TISSUE SPECIFICITY: Expressed in all tissues examined. PTM: Phosphorylated and activated by MAP kinase. SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain. |
| Molecular Weight | 45 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |