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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1584 |
| Description | Anti-Interleukin-2 (IL-2) Antibody, clone 3F9 |
| Alternate Names |
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| Background Information | Interleukin-2 (IL-2) is commonly considered a pro-inflammatory cytokine that stimulated T-cell-dependent immune reactions and control regulatory T cells or Tregs. Structurally, IL-2 is thought to have a 4-helix-bundle-type fold and uses a novel type I cytokine receptor (IL-21R) with the common gamma chain cytokine receptor. IL-2 also may intensify Th1-mediated diseases, including autoimmune arthritis and may also be interesting clinical targets for those diseases. IL-2 has also been implicated as a possible therapy for infection and cancer. Anti-tumor therapies that include a combination of recombinant IL-2 and recombinant interferon alpha have been promising in clinical studies. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Interleukin-2 (IL-2) using this Anti-Interleukin-2 (IL-2) Antibody, clone 3F9 validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant protein corresponding to human IL-2. |
| Clone | AB12.38.35C3.3F9 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. SUBCELLULAR LOCATION: Secreted. INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. PHARMACEUTICAL USE: Available under the name Proleukin (Chiron). Used in patients with renal cell carcinoma or metastatic melanoma. SEQUENCE SIMILARITIES: Belongs to the IL-2 family. SEQUENCE CAUTION: The sequence AAA59140.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~15 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in lysate from cells expressing human IL-2 recombinant protein. Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human IL-2 recombinant protein. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |