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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1550 |
| Description | Anti-SC-35 Antibody, clone 1SC-4F11 |
| Alternate Names |
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| Background Information | SC-35 is also known as Splicing factor SC35 and Splicing factor, arginine/serine-rich 2. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SC-35 is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is still widely disputed. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SC3-35 is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. SC-35 is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Unpurified mouse monoclonal IgG1κ preservative-free ascites. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SC35 in NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to human SC35. |
| Epitope | Unknown |
| Clone | clone 1SC-4F11 |
| Host | Mouse |
| Specificity | This antibody recognizes SC35. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5- and 3-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5- and 3-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5-AGSAGAGTA-3 (S=C or G) or 5-GTTCGAGTA-3. Can bind to beta-globin mRNA and commit it to the splicing pathway. SUBUNIT STRUCTURE: In vitro, binds SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SFRS2IP. SUBCELLULAR LOCATION: Nucleus. PTM: Extensively phosphorylated on serine residues in the RS domain. SEQUENCE SIMLARITIES: Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain. |
| Molecular Weight | ~ 35 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |