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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1545 |
| Description | Anti-RARα Antibody, clone 9α-9A6 |
| Alternate Names |
|
| Background Information | Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human RARα. |
| Epitope | Unknown |
| Clone | clone 9α-9A6 |
| Host | Mouse |
| Specificity | This antibody recognizes RARα. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Homology to mouse and rat is 91%. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression. SIZE: 462 amino acids; 50771 Da SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner. SUBCELLULAR LOCATION: Nucleus. DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain. |
| Molecular Weight | 58 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HL60 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |