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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1488 |
| Description | Anti-HIRA Antibody, clone WC119 |
| Alternate Names |
|
| Background Information | HIRA (TUP1 like enhancer of split protein 1) is a protein localized primarily in the nucleus. It functions as a histone chaparone to preferentially place histone H3.3 on nucleosome. It is broadly expressed in a variety of tissues and is expressed during embryogenesis. Data suggests HIRA plays a part in mechanisms of transcriptional regulation similar to that played by yeast HIR1 and HIR2 together. The HIRA protein has been shown to interact with HIRIP3, HIRIP5, histones H2B and H4, and PAX3. HIRA may play a part in the etiology of the DiGeorge syndrome (DGS), a developmental disorder. The clinical features of this disease include absence or hypoplasia of the thymus and parathyroid glands, cardiovascular malformations, facial dysplasia, a cleft palate and mental retardation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-HIRA Antibody, clone WC119 is a Mouse Monoclonal Antibody for detection of HIRA also known as HIR (histone cell cycle regulation defective) homolog & has been validated in WB & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Hall, C., et al. (2001). Molecular and Cellular Biology. 21(5):1854-1865.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human HIRA. |
| Epitope | Unknown |
| Clone | WC119 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes HIRA. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. SUBUNIT STRUCTURE: Interacts with histone H3F3B, PAX3 and PAX7 By similarity. Interacts with CCNA1, HIRIP3, NFU1/HIRIP5 and histone H2B. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1. SUBCELLULAR LOCATION: Nucleus. Note: Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. TISSUE SPECIFICTY: Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. Ref.6 DEVELOPMENTAL STAGE: Expressed during embryogenesis. PTM: Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. INVOLVEMENT IN DISEASE: May play a part in the etiology of the DiGeorge syndrome (DGS), a developmental disorder due to an abnormal development of the third and fourth pharyngeal pouches. The clinical features include absence or hypoplasia of the thymus and parathyroid glands, cardiovascular malformations, facial dysplasia, a cleft palate and mental retardation. SEQUENCE SIMILARITIES: Belongs to the WD repeat HIR1 family. Contains 8 WD repeats. |
| Molecular Weight | ~ 112 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 0.44 µg/ml of this antibody detected HIRA on 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |