Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-037 |
| Trade Name |
|
| Description | Anti-RalB Antibody, clone 25 |
| Alternate Names |
|
| Background Information | RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide of the C-terminus of RalB |
| Epitope | C-terminus |
| Clone | Clone 25 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody is specific for RalB and does not detect RalA. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, and Rat. Other species have not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | SUBUNIT STRUCTURE: Interacts with EXOC8. Interacts with RALBP1 via its effector domain. Ref.6 Ref.7 SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Ras family. |
| Molecular Weight | 24 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on HEK293 or NIH/3T3 cell lysates. Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |