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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB874 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5 |
| Alternate Names |
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| Background Information | Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Detect Papillomavirus using this Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5 validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: Reactive with a 16.5-18 kDa band in reduced westerns of overexpressing cells lines corresponding to HPV E6 protein in cells derived from human cervical carcinoma. {Banks, et al 1987} Immunohistochemistry: Shows reactivity on formalin fixed tissue. Dilute with buffer pH 7.5-8.0 to desired working volumes. For extensive dilution, protein containing or other stabilizing medium should be used. Immunopreciptiation: Immunoprecipiates 16.5 kDa band from human cervical carcinoma extracts {Banks, et al, 1987}. Final working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | C1P5 was made against HPV type 18 E6 protein produced in bacteria. |
| Epitope | 16, 18 E6 protein |
| Clone | C1P5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Human papilloma virus type 18 early E6 protein. The antibody cross reacts with E6 protein from HPV type 16. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| Molecular Weight | 16.5-18 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |