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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB8560 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 |
| Product Information | |
|---|---|
| Format | Ascites |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF. |
| Key Applications |
|
| Application Notes | IFA at 1:100-1:400. Optimal working dilutions must be determined by end user. SUGGESTED IFA PROTOCOL: Substrate slides: 1. Scrape and spot cells onto microscope slide wells. 2. Air Dry. 3. Fix in cold acetone for 10 minutes at +4°C. Stain: 1. Add 15 μL diluted polio antibody. 2. Incubate at +37°C for 30 minutes in a humid chamber. 3. Rinse in PBS for 5 minutes; repeat with fresh PBS. 4. Add 15 μL diluted labeled antibody. 5. Repeat steps 2 and 3 6. Mount with buffered glycerol. |
| Biological Information | |
|---|---|
| Clone | 583-G8-G2-A4 |
| Host | Mouse |
| Specificity | Reacts with the Poliovirus 1. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |