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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB8419 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227 |
| Alternate Names |
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| Background Information | Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cccDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signaling transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, WB & IC. |
| Key Applications |
|
| Application Notes | ELISA, immunoblotting and immunocytochemistry staining of infected liver tissues and HBV-transfected hepatoma cells. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Peptide cooresponding to aa90-115 of h HBV-X protein |
| Epitope | X-Protein, aa 90-115 |
| Clone | 227 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with Hbx-protein (trans-activator X gene product) of hepatitis B virus (epitope recognized - amino acid positions 90-115). SPECIES REACTIVITIES: Reacts only with HBV-infected human cells. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |