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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IF, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB810R |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Cytomegalovirus Antibody, clone 8B1.2 |
| Alternate Names |
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| Background Information | Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours. After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-Cytomegalovirus Antibody, clone 8B1.2 detects level of Cytomegalovirus & has been published & validated for use in ELISA, FC, IF, IH(P) & WB. |
| Key Applications |
|
| Application Notes | ELISA Western Blot: 1:200. Recognizes approximately a 70kDa band. Immunofluorescence: 1:200-1:400. Immunohistochemistry 1:200-1:800. Works on paraffin embedded tissue sections. FACS Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC). |
| Clone | 8B1.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| Molecular Weight | 68-72 kDa |
| Product Usage Statements | |
|---|---|
| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |