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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H, R, Rb | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1940 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Fibronectin Antibody, cellular, clone DH1 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | In PBS with 1% BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunoblotting at 1:1,000 Indirect immunohistochemistry at 1:200-1:400 A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996). Final working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified fibronectin from Human A8387 fibrosarcoma cells. |
| Epitope | cellular |
| Clone | DH1 |
| Host | Mouse |
| Specificity | Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at +2-8°C for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |